ELISA/assay

Bovine Tissue type Plasminogen Activator ELISA Kit

MBS740322

48-Strip-Wells

470 USD

ELISA Kit

Bovine Tissue type Plasminogen Activator ELISA Kit

Tissue type Plasminogen Activator

tissue-type plasminogen activator; Tissue-type plasminogen activator; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); plasminogen activator, tissue; INN: Alteplase; INN: ReteplaseCleaved into the following 2 chains:Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B

tPA

PLAT; PLAT; TPA; T-PA; t-PA; t-plasminogen activator; tPA

TPA_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of tPA. No significant cross-reactivity or interference between tPA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between tPA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This tPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine tPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: tPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-tPA antibody and an tPA-HRP conjugate. The assay sample and buffer are incubated together with tPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the tPA concentration since tPA from samples and tPA-HRP conjugate compete for the anti-tPA antibody binding site. Since the number of sites is limited, as more sites are occupied by tPA from the sample, fewer sites are left to bind tPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The tPA concentration in each sample is interpolated from this standard curve.

190032

AAA60111.1

44,373 Da

Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); Endochondral Ossification Pathway (198812); Hemostasis Pathway (106028); Selenium Pathway (198825); Senescence And Autophagy Pathway (198780); Signal Transduction Pathway (477114)

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.21.68; Secreted; Protease; Motility/polarity/chemotaxis; Vesicle; Secreted, signal peptide; Cytoskeletal. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule. Molecular Function: protein binding; serine-type endopeptidase activity. Biological Process: negative regulation of proteolysis; response to peptide hormone stimulus; synaptic transmission, glutamatergic; response to cAMP; smooth muscle cell migration; protein modification process; regulation of synaptic plasticity; platelet-derived growth factor receptor signaling pathway; proteolysis; fibrinolysis; response to hypoxia; blood coagulation; transmembrane transport; plasminogen activation. Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator

48-Strip-Wells

470 USD

96-Strip-Wells

675