Porcine Occludin ELISA Kit | MyBioSource MBS740246 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Occludin ELISA Kit

catalog :

MBS740246

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS740246

products type :

ELISA Kit

products full name :

Porcine Occludin ELISA Kit

products short name :

[Occludin]

other names :

[occludin; Occludin; occludin; tight junction protein occludin; phosphatase 1, regulatory subunit 115; occludin]

products gene name :

[OCLN]

other gene names :

[OCLN; OCLN; BLCPMG; PPP1R115]

uniprot entry name :

OCLN_HUMAN

reactivity :

Porcine

specificity :

This assay has high sensitivity and excellent specificity for detection of OCLN. No significant cross-reactivity or interference between OCLN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OCLN and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Signal Transduction

products description :

Intended Uses This OCLN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine OCLN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: OCLN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OCLN antibody and an OCLN-HRP conjugate. The assay sample and buffer are incubated together with OCLN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OCLN concentration since OCLN from samples and OCLN-HRP conjugate compete for the anti-OCLN antibody binding site. Since the number of sites is limited, as more sites are occupied by OCLN from the sample, fewer sites are left to bind OCLN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OCLN concentration in each sample is interpolated from this standard curve.

ncbi gi num :

1322282

ncbi acc num :

AAB00195.1

ncbi mol weight :

8,175 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (672459); Pathogenic Escherichia Coli Infection Pathway (516); TGF-beta Receptor Signaling Pathway (138024)

ncbi summary :

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

uniprot summary :

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 5q13.1. Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; intercellular junction; cytoplasmic vesicle; cytosol; cell junction. Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity. Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis. Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage