Monkey Apolipoprotein C3 ELISA Kit | MyBioSource MBS740170 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Apolipoprotein C3 ELISA Kit

catalog :

MBS740170

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS740170

products type :

ELISA Kit

products full name :

Monkey Apolipoprotein C3 ELISA Kit

products short name :

[Apolipoprotein C3]

other names :

[apolipoprotein C-III; Apolipoprotein C-III; apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3; apolipoprotein C-III; Apolipoprotein C3]

products gene name :

[APOC3]

other gene names :

[APOC3; APOC3; HALP2; APOCIII; Apo-CIII; ApoC-III]

uniprot entry name :

APOC3_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of ApoC3. No significant cross-reactivity or interference between ApoC3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoC3 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 50-1000ng/mL. Sensitivity: 1.0ng/mL

products categories :

Cardiovascular

products description :

Intended Uses: This ApoC3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ApoC3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ApoC3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoC3 antibody and an ApoC3-HRP conjugate. The assay sample and buffer are incubated together with ApoC3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoC3 concentration since ApoC3 from samples and ApoC3-HRP conjugate compete for the anti-ApoC3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoC3 from the sample, fewer sites are left to bind ApoC3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoC3 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4557323

ncbi acc num :

NP_000031.1

ncbi gb acc num :

NM_000040.1

ncbi mol weight :

10,852 Da

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

ncbi summary :

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

uniprot summary :

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family. Protein type: Lipid-binding; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: extracellular space; chylomicron; early endosome; extracellular region. Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity. Biological Process: response to drug; phototransduction, visible light; cholesterol metabolic process; response to peptide hormone stimulus; regulation of Cdc42 protein signal transduction; lipoprotein metabolic process; cholesterol efflux; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol metabolic process; negative regulation of lipid catabolic process; phospholipid efflux; lipoprotein transport; triacylglycerol catabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; inflammatory response; retinoid metabolic process; triacylglycerol mobilization; negative regulation of lipid metabolic process; response to nutrient; negative regulation of fatty acid biosynthetic process. Disease: Apolipoprotein C-iii Deficiency

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage