ELISA/assay

Goat S100 Calcium Binding Protein A7 ELISA Kit

MBS740147

48-Strip-Wells

470 USD

ELISA Kit

Goat S100 Calcium Binding Protein A7 ELISA Kit

S100 Calcium Binding Protein A7

S100A7; Protein S100-A7; Psoriasin; S100 calcium-binding protein A7

S100A7

S100A7; PSOR1; S100A7C

S10A7_HUMAN

Goat

This assay has high sensitivity and excellent specificity for detection of S100A7. No significant cross-reactivity or interference between S100A7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A7 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This S100A7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat S100A7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: S100A7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100A7 antibody and an S100A7-HRP conjugate. The assay sample and buffer are incubated together with S100A7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A7 concentration since S100A7 from samples and S100A7-HRP conjugate compete for the anti-S100A7 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A7 from the sample, fewer sites are left to bind S100A7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A7 concentration in each sample is interpolated from this standard curve.

49457324

CAG46961.1

P31151

11,471 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

S100A7: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known. [provided by RefSeq, Jul 2008]. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: focal adhesion; endoplasmic reticulum; cytoplasm; extracellular region; nucleus; cytosol. Molecular Function: RAGE receptor binding; protein binding; zinc ion binding; calcium ion binding. Biological Process: keratinocyte differentiation; sequestering of metal ion; response to reactive oxygen species; epidermis development; innate immune response; response to lipopolysaccharide; angiogenesis; defense response to Gram-negative bacterium

48-Strip-Wells

470 USD

96-Strip-Wells

675