ELISA/assay

Sheep Platelet Factor 4 ELISA Kit

MBS740037

48-Strip-Wells

470 USD

ELISA Kit

Sheep Platelet Factor 4 ELISA Kit

Platelet Factor 4

platelet factor 4; Platelet factor 4; platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; platelet factor 4; C-X-C motif chemokine 4; Iroplact; Oncostatin-ACleaved into the following chain:Platelet factor 4, short form

PF4

PF4; PF4; PF-4; CXCL4; SCYB4; CXCL4; SCYB4; PF-4

PLF4_HUMAN

Sheep

This assay has high sensitivity and excellent specificity for detection of PF-4. No significant cross-reactivity or interference between PF-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PF-4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PF-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PF-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: PF-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PF-4 antibody and an PF-4-HRP conjugate. The assay sample and buffer are incubated together with PF-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PF-4 concentration since PF-4 from samples and PF-4-HRP conjugate compete for the anti-PF-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PF-4 from the sample, fewer sites are left to bind PF-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF-4 concentration in each sample is interpolated from this standard curve.

4505733

NP_002610.1

NM_002619.3

10,845 Da

CXCR3-mediated Signaling Events Pathway (138011); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Common Pathway (106060); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057)

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Secreted; Secreted, signal peptide; Apoptosis; Motility/polarity/chemotaxis; Chemokine. Chromosomal Location of Human Ortholog: 4q12-q21. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity. Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; immune response; blood coagulation; inflammatory response; positive regulation of macrophage differentiation

48-Strip-Wells

470 USD

96-Strip-Wells

675