ELISA/assay

Porcine Glial Fibrillary Acidic Protein ELISA Kit

MBS740002

48-Strip-Wells

470 USD

ELISA Kit

Porcine Glial Fibrillary Acidic Protein ELISA Kit

Glial Fibrillary Acidic Protein

glial fibrillary acidic protein; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein

GFAP

GFAP; GFAP; GFAP

GFAP_HUMAN

Porcine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Neurobiology

Intended Uses: This GFAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GFAP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GFAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GFAP antibody and an GFAP-HRP conjugate. The assay sample and buffer are incubated together with GFAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GFAP concentration since GFAP from samples and GFAP-HRP conjugate compete for the anti-GFAP antibody binding site. Since the number of sites is limited, as more sites are occupied by GFAP from the sample, fewer sites are left to bind GFAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GFAP concentration in each sample is interpolated from this standard curve.

251802

AAB22581.1

49,508 Da

Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (530744); Signal Transduction Pathway (477114); Signaling By ERBB4 Pathway (530741); Spinal Cord Injury Pathway (739007)

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: membrane; cytoplasm; intermediate filament; cytosol. Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding. Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development. Disease: Alexander Disease

48-Strip-Wells

470 USD

96-Strip-Wells

675