ELISA/assay

Porcine Serum amyloid A ELISA Kit

MBS739949

48-Strip-Wells

470 USD

ELISA Kit

Porcine Serum amyloid A ELISA Kit

Serum amyloid A

serum amyloid A protein; serum amyloid A protein

SAA

SAA1; SAA

Porcine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ug/mL

Intended Uses: This SAA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SAA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: SAA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SAA antibody and an SAA-HRP conjugate. The assay sample and buffer are incubated together with SAA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAA concentration since SAA from samples and SAA-HRP conjugate compete for the anti-SAA antibody binding site. Since the number of sites is limited, as more sites are occupied by SAA from the sample, fewer sites are left to bind SAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAA concentration in each sample is interpolated from this standard curve.

93352563

NP_001035288.1

NM_001040198.1

48-Strip-Wells

470 USD

96-Strip-Wells

675