Canine Diamine Oxidase ELISA Kit | MyBioSource MBS739902 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Diamine Oxidase ELISA Kit

catalog :

MBS739902

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS739902

products type :

ELISA Kit

products full name :

Canine Diamine Oxidase ELISA Kit

products short name :

Diamine Oxidase

other names :

amiloride-sensitive amine oxidase; Amiloride-sensitive amine oxidase [copper-containing]; amiloride-sensitive amine oxidase [copper-containing]; amiloride-sensitive amine oxidase [copper-containing]; histaminase; diamine oxidase; kidney amine oxidase; amiloride-binding protein 1; amine oxidase copper domain-containing protein 1; amiloride binding protein 1 (amine oxidase (copper-containing)); amine oxidase, copper containing 1; Amiloride-binding protein 1; Amine oxidase copper domain-containing protein 1; Histaminase; Kidney amine oxidase; KAO

products gene name :

DAO

other gene names :

AOC1; AOC1; ABP; DAO; KAO; ABP1; DAO1; ABP1; DAO1; DAO; Diamine oxidase; KAO

uniprot entry name :

AOC1_HUMAN

reactivity :

Canine

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

other info2 :

Intended Uses: This DAO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine DAO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: DAO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DAO antibody and an DAO-HRP conjugate. The assay sample and buffer are incubated together with DAO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAO concentration since DAO from samples and DAO-HRP conjugate compete for the anti-DAO antibody binding site. Since the number of sites is limited, as more sites are occupied by DAO from the sample, fewer sites are left to bind DAO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAO concentration in each sample is interpolated from this standard curve.

ncbi gi num :

440918691

ncbi acc num :

NP_001259001.1

ncbi gb acc num :

NM_001272072.1

ncbi mol weight :

87,239 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Histidine Metabolism Pathway (82958); Histidine Metabolism Pathway (324); Tryptophan Metabolism Pathway (82964); Tryptophan Metabolism Pathway (198850); Tryptophan Metabolism Pathway (332); Histamine Degradation Pathway (545334); Histamine Degradation Pathway (139087)

ncbi summary :

This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

uniprot summary :

ABP1: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. Belongs to the copper/topaquinone oxidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Amino Acid Metabolism - tryptophan; EC 1.4.3.22; Oxidoreductase; Amino Acid Metabolism - histidine; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 7q36.1. Cellular Component: extracellular space; tight junction; plasma membrane; peroxisome. Molecular Function: amine oxidase activity; heparin binding; protein homodimerization activity; copper ion binding; zinc ion binding; protein complex binding; cation channel activity; calcium ion binding; receptor activity; drug binding; sodium channel activity; quinone binding. Biological Process: response to drug; response to antibiotic; amine metabolic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage