Bovine Cathelicidin Antimicrobial Peptide ELISA Kit | MyBioSource MBS739799 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Cathelicidin Antimicrobial Peptide ELISA Kit

catalog :

MBS739799

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS739799

products type :

ELISA Kit

products full name :

Bovine Cathelicidin Antimicrobial Peptide ELISA Kit

products short name :

[Cathelicidin Antimicrobial Peptide]

other names :

[CAMP, partial; Cathelicidin antimicrobial peptide; 18 kDa cationic antimicrobial protein; CAP-18; hCAP-18]

products gene name :

[CAMP]

other gene names :

[CAMP; CAP18; FALL39; HSD26; CAP-18; hCAP-18]

uniprot entry name :

CAMP_HUMAN

reactivity :

Bovine

specificity :

This assay has high sensitivity and excellent specificity for detection of CAMP. No significant cross-reactivity or interference between CAMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAMP and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 50-1000pg/ml. Sensitivity: 1.0pg/ml

products categories :

Microbiology

products description :

Intended Uses: This CAMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CAMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAMP antibody and an CAMP-HRP conjugate. The assay sample and buffer are incubated together with CAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMP concentration since CAMP from samples and CAMP-HRP conjugate compete for the anti-CAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMP from the sample, fewer sites are left to bind CAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMP concentration in each sample is interpolated from this standard curve.

ncbi gi num :

49456877

ncbi acc num :

CAG46759.1

uniprot acc num :

P49913

ncbi mol weight :

19,301 Da

ncbi pathways :

Disease Pathway (530764); Integrated Pancreatic Cancer Pathway (711360); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (645267); Phagosomal Maturation (early Endosomal Stage) Pathway (645268); SREBP Signalling Pathway (219802); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

ncbi summary :

This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. [provided by RefSeq, Sep 2014]

uniprot summary :

CAMP: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. Belongs to the cathelicidin family. Protein type: Secreted, signal peptide; Cell cycle regulation; Vesicle; Secreted. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: extracellular space; specific granule; cell projection; cell wall; cytosol. Molecular Function: protein binding. Biological Process: interaction with host; defense response to Gram-positive bacterium; positive regulation of angiogenesis; innate immune response in mucosa; killing by host of symbiont cells; defense response to bacterium; positive regulation of cell proliferation; antibacterial humoral response; positive regulation of protein amino acid phosphorylation; defense response to Gram-negative bacterium

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage