product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Bovine Monocyte Chemotactic Protein 3 ELISA Kit
catalog :
MBS739771
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS739771
products type :
ELISA Kit
products full name :
Bovine Monocyte Chemotactic Protein 3 ELISA Kit
products short name :
Monocyte Chemotactic Protein 3
other names :
monocyte chemotactic protein-3; C-C motif chemokine 7; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; NC28; Small-inducible cytokine A7
products gene name :
MCP-3
other gene names :
CCL7; MCP3; SCYA6; SCYA7; MCP-3
uniprot entry name :
CCL7_HUMAN
reactivity :
Bovine
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This MCP-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine MCP-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: MCP-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCP-3 antibody and an MCP-3-HRP conjugate. The assay sample and buffer are incubated together with MCP-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCP-3 concentration since MCP-3 from samples and MCP-3-HRP conjugate compete for the anti-MCP-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MCP-3 from the sample, fewer sites are left to bind MCP-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP-3 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
313708
ncbi acc num :
CAA51055.1
uniprot acc num :
P80098
ncbi mol weight :
11,200 Da
uniprot summary :
CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q11.2-q12. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity; CCR2 chemokine receptor binding. Biological Process: cellular calcium ion homeostasis; regulation of cell shape; monocyte chemotaxis; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; cytoskeleton organization and biogenesis; immune response; signal transduction; inflammatory response; chemotaxis; positive regulation of cell migration
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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