ELISA/assay

Sheep High sensitivity C-Reactive Protein ELISA Kit

MBS739718

48-Strip-Wells

470 USD

ELISA Kit

Sheep High sensitivity C-Reactive Protein ELISA Kit

High sensitivity C-Reactive Protein

CRP; C-reactive protein; C-reactive protein; pentraxin 1; C-reactive protein, pentraxin-related

Hs-CRP

CRP; CRP; PTX1; PTX1

CRP_HUMAN

Sheep

This assay has high sensitivity and excellent specificity for detection of CRP-HS. No significant cross-reactivity or interference between CRP-HS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRP-HS and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This CRP-HS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep CRP-HS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: CRP-HS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRP-HS antibody and an CRP-HS-HRP conjugate. The assay sample and buffer are incubated together with CRP-HS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRP-HS concentration since CRP-HS from samples and CRP-HS-HRP conjugate compete for the anti-CRP-HS antibody binding site. Since the number of sites is limited, as more sites are occupied by CRP-HS from the sample, fewer sites are left to bind CRP-HS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRP-HS concentration in each sample is interpolated from this standard curve.

17975775

AAL48218.2

10,415 Da

Classical Antibody-mediated Complement Activation Pathway (106409); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Selenium Pathway (198825)

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q23.2. Cellular Component: extracellular space; growth cone; extracellular region; filopodium. Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; cholesterol binding; low-density lipoprotein binding; virion binding; calcium ion binding. Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; response to lead ion; response to hypoxia; acute-phase response; opsonization; inflammatory response; complement activation, classical pathway; aging

48-Strip-Wells

470 USD

96-Strip-Wells

675