ELISA/assay

Porcine Dihydrotestosterone ELISA Kit

MBS739627

48-Strip-Wells

470 USD

ELISA Kit

Porcine Dihydrotestosterone ELISA Kit

Dihydrotestosterone

D-hydantoinase/dihydropyrimidinase Dht; D-hydantoinase/dihydropyrimidinase Dht; D-hydantoinase/dihydropyrimidinase Dht; D-hydantoinase/dihydropyrimidinase Dht

DHT

dht; dht; RLO149_c023310

F7ZBB5_ROSLO

Porcine

This assay has high sensitivity and excellent specificity for detection of DHT. No significant cross-reactivity or interference between DHT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DHT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 nmol/L.

Intended Uses: This DHT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine DHT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: DHT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DHT antibody and an DHT-HRP conjugate. The assay sample and buffer are incubated together with DHT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DHT concentration since DHT from samples and DHT-HRP conjugate compete for the anti-DHT antibody binding site. Since the number of sites is limited, as more sites are occupied by DHT from the sample, fewer sites are left to bind DHT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHT concentration in each sample is interpolated from this standard curve.

339503844

YP_004691264.1

NC_015730.1

52,872 Da

Metabolic Pathways (377010); Pantothenate And CoA Biosynthesis Pathway (376997); Pantothenate And CoA Biosynthesis Pathway (401); Pyrimidine Metabolism Pathway (376946); Pyrimidine Metabolism Pathway (309); Beta-Alanine Metabolism Pathway (376962); Beta-Alanine Metabolism Pathway (335)

48-Strip-Wells

470 USD

96-Strip-Wells

675