Rat Apolipoprotein L1 ELISA Kit | MyBioSource MBS739571 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Apolipoprotein L1 ELISA Kit

catalog :

MBS739571

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS739571

products type :

ELISA Kit

products full name :

Rat Apolipoprotein L1 ELISA Kit

products short name :

Apolipoprotein L1

other names :

apolipoprotein L1 isoform a; Apolipoprotein L1; apolipoprotein L1; apolipoprotein L, 1; Apolipoprotein L; Apo-L; ApoL; Apolipoprotein L-I; ApoL-I

products gene name :

APOL1

other gene names :

APOL1; APOL1; APOL; APO-L; FSGS4; APOL-I; APOL; Apo-L; ApoL; ApoL-I

uniprot entry name :

APOL1_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of ApoL1. No significant cross-reactivity or interference between ApoL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoL1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

other info2 :

Intended Uses: This ApoL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ApoL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

Principle of the assay: ApoL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoL1 antibody and an ApoL1-HRP conjugate. The assay sample and buffer are incubated together with ApoL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoL1 concentration since ApoL1 from samples and ApoL1-HRP conjugate compete for the anti-ApoL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoL1 from the sample, fewer sites are left to bind ApoL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoL1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

211938440

ncbi acc num :

NP_001130012.1

ncbi gb acc num :

NM_001136540.1

ncbi mol weight :

42,158 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600)

ncbi summary :

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

uniprot summary :

APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Channel, chloride; Secreted; Lipid-binding; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: extracellular space; extracellular region; intrinsic to membrane. Molecular Function: chloride channel activity; protein binding; lipid binding. Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; killing of cells of another organism; cytolysis; innate immune response; lipoprotein metabolic process; chloride transport; lipid transport. Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage