Monkey Galectin 3 ELISA Kit | MyBioSource MBS739544 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Galectin 3 ELISA Kit

catalog :

MBS739544

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS739544

products type :

ELISA Kit

products full name :

Monkey Galectin 3 ELISA Kit

products short name :

Galectin 3

other names :

galectin-3 isoform 2; Galectin-3; galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; lectin, galactoside-binding, soluble, 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen

products gene name :

GAL3

other gene names :

LGALS3; LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2; MAC2; Gal-3; CBP 35; GALBP

uniprot entry name :

LEG3_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of GAL-3. No significant cross-reactivity or interference between GAL-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAL-3 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1ng/mL

products categories :

Cell Biology

products description :

Intended Uses: This GAL-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GAL-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GAL-3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GAL-3. Standards or samples are then added to the microtiter plate wells and GAL-3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GAL-3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GAL-3 are added to each well to "sandwich" the GAL-3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GAL-3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL-3 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

294345475

ncbi acc num :

NP_001170859.1

ncbi gb acc num :

NM_001177388.1

ncbi mol weight :

26,152 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Hedgehog Signaling Events Mediated By Gli Proteins Pathway (137912); Immune System Pathway (106386); Innate Immune System Pathway (106387); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

uniprot summary :

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages. Protein type: Motility/polarity/chemotaxis; Cell surface; Extracellular matrix. Chromosomal Location of Human Ortholog: 14q22.3. Cellular Component: extracellular matrix; spliceosome; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane. Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity. Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; positive chemotaxis; epithelial cell differentiation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage