ELISA/assay

Human Angiotensin 2 Receptor 1 Antibody ELISA Kit

MBS739430

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Angiotensin 2 Receptor 1 Antibody ELISA Kit

Angiotensin 2 Receptor 1 Antibody

Angiotensin II receptor, type 1; Type-1 angiotensin II receptor; type-1 angiotensin II receptor; type-1B angiotensin II receptor; angiotensin II receptor, type 1; AT1AR; AT1BR; Angiotensin II type-1 receptor; AT1

AT2R1

AGTR1; AGTR1; AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B; AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1

AGTR1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of A2R1Ab. No significant cross-reactivity or interference between A2R1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between A2R1Ab and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL.

Signal Transduction

Intended Uses: This A2R1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human A2R1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay A2R1Ab ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Angiotensin 2 Receptor 1antigen. Standards or samples are then added to the microtiter plate wells and A2R1Ab if present, will bind to the Angiotensin 2 Receptor 1 antigen pre-coated wells. In order to quantitatively determine the amount of A2R1Ab present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated A2R1Ab IgG is added to each well to "sandwich" the A2R1Ab immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain A2R1Ab and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A2R1Ab concentration in each sample is interpolated from this standard curve.

46250427

AAH68494.1

41,061 Da

ACE Inhibitor Pathway (198763); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arf6 Signaling Events Pathway (138034); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548)

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]

AT1: receptor for angiotensin II. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. Mediates the major cardiovascular effects of angiotensin II. May play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR. Chromosomal Location of Human Ortholog: 3q24. Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane. Molecular Function: protein binding; angiotensin type II receptor activity; protein heterodimerization activity; angiotensin type I receptor activity; bradykinin receptor binding. Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of vasodilation; positive regulation of cellular protein metabolic process; renin-angiotensin regulation of aldosterone production; calcium-mediated signaling; regulation of systemic arterial blood pressure by renin-angiotensin; Rho protein signal transduction; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; renin-angiotensin regulation of blood vessel size; regulation of vasoconstriction; regulation of inflammatory response; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of cell growth; kidney development; positive regulation of phospholipase A2 activity; positive regulation of NAD(P)H oxidase activity; positive regulation of inflammatory response. Disease: Renal Tubular Dysgenesis; Hypertension, Essential

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675