ELISA/assay

Canine Carbonic Anhydrase I ELISA Kit

MBS739410

48-Strip-Wells

470 USD

ELISA Kit

Canine Carbonic Anhydrase I ELISA Kit

Carbonic Anhydrase I

CA1 protein; Carbonic anhydrase 1; carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; epididymis secretory protein Li 11; carbonic anhydrase I; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I

CA1

CA1; CA1; CAB; CA-I; Car1; HEL-S-11; CAB; CA-I

CAH1_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of CA-1. No significant cross-reactivity or interference between CA-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CA-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CA-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CA-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CA-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CA-1 antibody and an CA-1-HRP conjugate. The assay sample and buffer are incubated together with CA-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA-1 concentration since CA-1 from samples and CA-1-HRP conjugate compete for the anti-CA-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA-1 from the sample, fewer sites are left to bind CA-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA-1 concentration in each sample is interpolated from this standard curve.

20380766

AAH27890.1

28,870 Da

C-MYB Transcription Factor Network Pathway (138073); Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway (645347); Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway (645348); Metabolism Pathway (477135); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416); O2/CO2 Exchange In Erythrocytes Pathway (645346); Reversible Hydration Of Carbon Dioxide Pathway (645352)

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family. Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen. Chromosomal Location of Human Ortholog: 8q21.2. Cellular Component: cytosol. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; one-carbon compound metabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675