Sheep Osteocalcin ELISA Kit | MyBioSource MBS739395 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sheep Osteocalcin ELISA Kit

catalog :

MBS739395

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS739395

products type :

ELISA Kit

products full name :

Sheep Osteocalcin ELISA Kit

products short name :

Osteocalcin

other names :

osteocalcin preproprotein; Osteocalcin; osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

products gene name :

other gene names :

BGLAP; BGLAP; OC; BGP; OCN; BGP

uniprot entry name :

OSTCN_HUMAN

reactivity :

Sheep

specificity :

This assay has high sensitivity and excellent specificity for detection of OC. No significant cross-reactivity or interference between OC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OC and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1ng/mL.

other info2 :

Intended Uses: This OC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat OC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

Principle of the assay: OC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OC antibody and an OC-HRP conjugate. The assay sample and buffer are incubated together with OC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OC concentration since OC from samples and OC-HRP conjugate compete for the anti-OC antibody binding site. Since the number of sites is limited, as more sites are occupied by OC from the sample, fewer sites are left to bind OC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OC concentration in each sample is interpolated from this standard curve.

ncbi gi num :

40316933

ncbi acc num :

NP_954642.1

ncbi gb acc num :

NM_199173.4

ncbi mol weight :

10,963 Da

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); FGF Signaling Pathway (137989); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); M Phase Pathway (105812); Mitotic Anaphase Pathway (105818); Mitotic Metaphase And Anaphase Pathway (730317); Mitotic Prometaphase Pathway (105815); Notch-mediated HES/HEY Network Pathway (169347)

ncbi summary :

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

uniprot summary :

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon. Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity. Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; response to ethanol; regulation of osteoclast differentiation; response to vitamin D; response to vitamin K; response to zinc ion; response to mechanical stimulus; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; regulation of bone mineralization; skeletal development

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage