ELISA/assay

Monkey Matrix Metalloproteinase 3 ELISA Kit

MBS739370

48-Strip-Wells

470 USD

ELISA Kit

Monkey Matrix Metalloproteinase 3 ELISA Kit

Matrix Metalloproteinase 3

MMP-3, partial; MMP-3; stromelysin-1; metalloproteinase 3 receptor; matrix metallopeptidase 3 (stromelysin 1, progelatinase)<; MMP-3

MMP3

MMP3; bmmp-3

Q9N283_BOVIN

Monkey

This assay has high sensitivity and excellent specificity for detection of MMP3. No significant cross-reactivity or interference between MMP3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This MMP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey MMP3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: MMP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP3 antibody and an MMP3-HRP conjugate. The assay sample and buffer are incubated together with MMP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP3 concentration since MMP3 from samples and MMP3-HRP conjugate compete for the anti-MMP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP3 from the sample, fewer sites are left to bind MMP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP3 concentration in each sample is interpolated from this standard curve.

8176609

BAA96388.1

16,673 Da

Matrix Metalloproteinases Pathway (198565); Rheumatoid Arthritis Pathway (200308); Rheumatoid Arthritis Pathway (200269); TNF Signaling Pathway (812258); TNF Signaling Pathway (813210); Transcriptional Misregulation In Cancer Pathway (522997); Transcriptional Misregulation In Cancer Pathway (522987)

48-Strip-Wells

470 USD

96-Strip-Wells

675