ELISA/assay

Canine Gamma Glutamyltransferase 1 ELISA Kit

MBS739358

48-Strip-Wells

470 USD

ELISA Kit

Canine Gamma Glutamyltransferase 1 ELISA Kit

Gamma Glutamyltransferase 1

GGT1; Gamma-glutamyltranspeptidase 1; gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase; gamma-glutamyltransferase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain

gGT1

GGT1; GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732; GGT; GGT 1

GGT1_HUMAN

Canine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

Intended Uses: This gammaGT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine gammaGT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This gammaGT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine gammaGT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: gammaGT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gammaGT-1 antibody and an gammaGT-1-HRP conjugate. The assay sample and buffer are incubated together with gammaGT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gammaGT-1 concentration since gammaGT-1 from samples and gammaGT-1-HRP conjugate compete for the anti-gammaGT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by gammaGT-1 from the sample, fewer sites are left to bind gammaGT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gammaGT-1 concentration in each sample is interpolated from this standard curve.

47678519

CAG30380.1

P19440

24,080 Da

Aflatoxin Activation And Detoxification Pathway (1016173); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (105698); Cyanoamino Acid Metabolism Pathway (82970); Cyanoamino Acid Metabolism Pathway (339); Eicosanoid Synthesis Pathway (198888); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973)

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: EC 3.4.19.13; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - selenoamino acid; EC 2.3.2.2; Transferase; EC 3.4.19.14; Other Amino Acids Metabolism - cyanoamino acid; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Membrane protein, integral. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: anchored to external side of plasma membrane; extracellular space; plasma membrane; integral to membrane. Molecular Function: protein binding; gamma-glutamyltransferase activity. Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; arachidonic acid metabolic process; spermatogenesis; zymogen activation. Disease: Glutathionuria

48-Strip-Wells

470 USD

96-Strip-Wells

675