ELISA/assay

Chicken Insulin like Growth Factor1 ELISA Kit

MBS739221

48-Strip-Wells

470 USD

ELISA Kit

Chicken Insulin like Growth Factor1 ELISA Kit

Insulin like Growth Factor1

insulin-like growth factor 1 isoform 3 preproprotein; Insulin-like growth factor I; insulin-like growth factor 1; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; insulin-like growth factor 1 (somatomedin C); Mechano growth factor; MGF; Somatomedin-C

IGF1

IGF1; IGF1; IGFI; IGF-I; IGF1A; IBP1; IGF-I; MGF

IGF1_HUMAN

Chicken

This assay has high sensitivity and excellent specificity for detection of IGF-1. No significant cross-reactivity or interference between IGF-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGF-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This IGF-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken IGF-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: IGF-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF-1 antibody and an IGF-1-HRP conjugate. The assay sample and buffer are incubated together with IGF-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF-1 concentration since IGF-1 from samples and IGF-1-HRP conjugate compete for the anti-IGF-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF-1 from the sample, fewer sites are left to bind IGF-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF-1 concentration in each sample is interpolated from this standard curve.

163659903

NP_001104755.1

NM_001111285.1

P05019

21,841 Da

Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198806); Apoptosis Pathway (198797); Diabetes Pathways (105902); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Disease Pathway (530764); Endochondral Ossification Pathway (198812)

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted. Chromosomal Location of Human Ortholog: 12q23.2. Cellular Component: insulin-like growth factor binding protein complex; extracellular space; plasma membrane; extracellular region. Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding. Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; glycolate metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of mitosis; positive regulation of protein import into nucleus, translocation; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; branching morphogenesis of a tube; response to heat; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; myoblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of glycogen biosynthetic process; positive regulation of smooth muscle cell migration; positive regulation of activated T cell proliferation; signal transduction; negative regulation of cell proliferation; platelet degranulation; glial cell differentiation; mammary gland development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; regulation of multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; cell proliferation; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; Ras protein signal transduction; blood vessel remodeling; positive regulation of Ras protein signal transduction; blood coagulation; cell motility; positive regulation of DNA replication. Disease: Insulin-like Growth Factor I Deficiency

48-Strip-Wells

470 USD

96-Strip-Wells

675