ELISA/assay

Porcine Cartilage Oligomeric Matrix Protein ELISA Kit

MBS739208

48-Strip-Wells

440 USD

ELISA Kit

Porcine Cartilage Oligomeric Matrix Protein ELISA Kit

[Cartilage Oligomeric Matrix Protein]

[ComP; ComP; ComP]

[COMP]

[comP]

O30583_9GAMM

Porcine

This assay has high sensitivity and excellent specificity for detection of COLI. No significant cross-reactivity or interference between COLI and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COLI and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.

Signal Transduction

Intended Uses: This COLI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig COLI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: COLI ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COLI antibody and an COLI-HRP conjugate. The assay sample and buffer are incubated together with COLI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COLI concentration since COLI from samples and COLI-HRP conjugate compete for the anti-COLI antibody binding site. Since the number of sites is limited, as more sites are occupied by COLI from the sample, fewer sites are left to bind COLI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COLI concentration in each sample is interpolated from this standard curve.

2618827

AAC45886.1

14,872 Da

Pilin-like competence factor, which is essential for natural transformation of the Gram-negative soil bacterium A.baylyi ADP1. Is not a subunit of the pilus structures. Likely functions as a major subunit of an oligomeric structure acting as a channel or pore mediating DNA translocation through the outer membrane and periplasm.

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415