Porcine Growth Associated Protein 43 ELISA Kit | MyBioSource MBS739175 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Growth Associated Protein 43 ELISA Kit

catalog :

MBS739175

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS739175

products type :

ELISA Kit

products full name :

Porcine Growth Associated Protein 43 ELISA Kit

products short name :

Growth Associated Protein 43

other names :

growth associated protein 43; Neuromodulin; neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43; growth associated protein 43; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46

products gene name :

GAP43

other gene names :

GAP43; GAP43; B-50; PP46

uniprot entry name :

NEUM_HUMAN

reactivity :

Porcine

specificity :

This assay has high sensitivity and excellent specificity for detection of GAP43. No significant cross-reactivity or interference between GAP43 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAP43 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1ng/mL

products categories :

Neurobiology

products description :

Intended Uses: This GAP43 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GAP43. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GAP43 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GAP43 antibody and an GAP43-HRP conjugate. The assay sample and buffer are incubated together with GAP43-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAP43 concentration since GAP43 from samples and GAP43-HRP conjugate compete for the anti-GAP43 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAP43 from the sample, fewer sites are left to bind GAP43-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAP43 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

119600000

ncbi acc num :

EAW79594.1

ncbi mol weight :

28,766 Da

ncbi pathways :

Glial Cell Differentiation Pathway (698758); N-cadherin Signaling Events Pathway (137919); Spinal Cord Injury Pathway (739007)

ncbi summary :

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface. Chromosomal Location of Human Ortholog: 3q13.31. Cellular Component: filopodium membrane; axon; cytoplasm; postsynaptic density; plasma membrane; cell junction. Molecular Function: calmodulin binding; protein binding. Biological Process: glial cell differentiation; cell fate commitment; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage