ELISA/assay

Monkey Interleukin 9 ELISA Kit

MBS739087

48-Strip-Wells

470 USD

ELISA Kit

Monkey Interleukin 9 ELISA Kit

Interleukin 9

interleukin-9; Interleukin-9; interleukin-9; cytokine P40; p40 cytokine; T-cell growth factor p40; p40 T-cell and mast cell growth factor; homolog of mouse T cell and mast cell growth factor 40; interleukin 9; Cytokine P40; T-cell growth factor P40

IL-9

IL9; IL9; P40; HP40; IL-9; IL-9

IL9_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of IL-9. No significant cross-reactivity or interference between IL-9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-9 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This IL-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IL-9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-9 antibody and an IL-9-HRP conjugate. The assay sample and buffer are incubated together with IL-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-9 concentration since IL-9 from samples and IL-9-HRP conjugate compete for the anti-IL-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-9 from the sample, fewer sites are left to bind IL-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-9 concentration in each sample is interpolated from this standard curve.

10834980

NP_000581.1

NM_000590.1

15,909 Da

Asthma Pathway (83120); Asthma Pathway (532); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL-9 Signaling Pathway (198883); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); TCR Signaling Pathway (198862)

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]

IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family. Protein type: Secreted; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space. Molecular Function: growth factor activity; interleukin-9 receptor binding; cytokine activity. Biological Process: positive regulation of cell proliferation; immune response; inflammatory response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process

48-Strip-Wells

470 USD

96-Strip-Wells

675