ELISA/assay

Canine Glypican 3 ELISA Kit

MBS739002

48-Strip-Wells

470 USD

ELISA Kit

Canine Glypican 3 ELISA Kit

Glypican 3

glypican-3 isoform 4; Glypican-3; glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan; glypican 3; GTR2-2; Intestinal protein OCI-5; MXR7Cleaved into the following chain:Secreted glypican-3

GPC3

GPC3; GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; OCI5

GPC3_HUMAN

Canine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GPC3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GPC3. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Neurobiology

Principle of the Assay: GPC3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPC3 antibody and an GPC3-HRP conjugate. The assay sample and buffer are incubated together with GPC3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPC3 concentration since GPC3 from samples and GPC3-HRP conjugate compete for the anti-GPC3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPC3 from the sample, fewer sites are left to bind GPC3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPC3 concentration in each sample is interpolated from this standard curve.

257471010

NP_001158091.1

NM_001164619.1

68,414 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Metabolism Pathway (645297); Glypican 3 Network Pathway (138084); Glypican Pathway (138083); HS-GAG Biosynthesis Pathway (645306); HS-GAG Degradation Pathway (645307)

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family. Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: Xq26.1. Cellular Component: extracellular space; lysosomal lumen; proteinaceous extracellular matrix; anchored to plasma membrane; Golgi lumen; integral to plasma membrane; plasma membrane. Molecular Function: heparan sulfate proteoglycan binding; protein binding. Biological Process: phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan metabolic process; negative regulation of peptidase activity; positive regulation of endocytosis; pathogenesis; osteoclast differentiation; embryonic hindlimb morphogenesis; body morphogenesis; bone mineralization; chondroitin sulfate metabolic process; positive regulation of glucose import; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; negative regulation of epithelial cell proliferation; anterior/posterior axis specification; negative regulation of growth; lung development. Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1

48-Strip-Wells

470 USD

96-Strip-Wells

675