ELISA/assay

Sheep Interleukin 23 ELISA Kit

MBS738988

48-Strip-Wells

470 USD

ELISA Kit

Sheep Interleukin 23 ELISA Kit

Interleukin 23

interleukin-23 subunit alpha; Interleukin-23 subunit alpha; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor; interleukin 23, alpha subunit p19; Interleukin-23 subunit p19; IL-23p19

IL-23

IL23A; IL23A; P19; SGRF; IL-23; IL-23A; IL23P19; SGRF; UNQ2498/PRO5798; IL-23 subunit alpha; IL-23-A; IL-23p19

IL23A_HUMAN

Sheep

This assay has high sensitivity and excellent specificity for detection of IL-23. No significant cross-reactivity or interference between IL-23 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-23 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This IL-23 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IL-23. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-23 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-23 antibody and an IL-23-HRP conjugate. The assay sample and buffer are incubated together with IL-23-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-23 concentration since IL-23 from samples and IL-23-HRP conjugate compete for the anti-IL-23 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-23 from the sample, fewer sites are left to bind IL-23-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-23 concentration in each sample is interpolated from this standard curve.

7706702

NP_057668.1

NM_016584.2

20,730 Da

ATF-2 Transcription Factor Network Pathway (138006); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL23-mediated Signaling Events Pathway (138000); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Pertussis Pathway (218111); Pertussis Pathway (218099)

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008]

IL23A: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Belongs to the IL-6 superfamily. Protein type: Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 12q13.3. Molecular Function: protein binding; cytokine activity; interleukin-23 receptor binding. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of T-helper 1 type immune response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; positive regulation of osteoclast differentiation; tissue remodeling; positive regulation of NK T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; positive regulation of NF-kappaB import into nucleus; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; positive regulation of interleukin-10 production; positive regulation of natural killer cell activation; positive regulation of tyrosine phosphorylation of Stat3 protein; T cell proliferation; positive regulation of T cell proliferation; inflammatory response; defense response to virus; positive regulation of interleukin-17 production; positive regulation of memory T cell differentiation; positive regulation of NK T cell activation; positive regulation of natural killer cell proliferation; positive regulation of tumor necrosis factor production; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; positive regulation of inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675