ELISA/assay

Canine Macrophage MigRation Inhibitory Factor ELISA Kit

MBS738733

48-Strip-Wells

470 USD

ELISA Kit

Canine Macrophage MigRation Inhibitory Factor ELISA Kit

Macrophage MigRation Inhibitory Factor

MIF; Macrophage migration inhibitory factor; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase

MIF

MIF; MIF; GIF; GLIF; MMIF; GLIF; MMIF; MIF; GIF

MIF_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of MIF. No significant cross-reactivity or interference between MIF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MIF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This MIF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MIF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: MIF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MIF antibody and an MIF-HRP conjugate. The assay sample and buffer are incubated together with MIF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIF concentration since MIF from samples and MIF-HRP conjugate compete for the anti-MIF antibody binding site. Since the number of sites is limited, as more sites are occupied by MIF from the sample, fewer sites are left to bind MIF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIF concentration in each sample is interpolated from this standard curve.

47678571

CAG30406.1

P14174

12,476 Da

Adipogenesis Pathway (198832); Phenylalanine Metabolism Pathway (82960); Phenylalanine Metabolism Pathway (327); Spinal Cord Injury Pathway (739007); Tyrosine Metabolism Pathway (82959); Tyrosine Metabolism Pathway (325)

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase. Protein type: Cell development/differentiation; Apoptosis; Cytokine; Amino Acid Metabolism - tyrosine; EC 5.3.2.1; EC 5.3.3.12; Isomerase; Amino Acid Metabolism - phenylalanine. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle. Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; chemoattractant activity; receptor binding. Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; carboxylic acid metabolic process; positive regulation of lipopolysaccharide-mediated signaling pathway; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of MAP kinase activity; cell proliferation; negative regulation of myeloid cell apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of fibroblast proliferation; cell surface receptor linked signal transduction; negative regulation of mature B cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cytokine secretion. Disease: Rheumatoid Arthritis, Systemic Juvenile

48-Strip-Wells

470 USD

96-Strip-Wells

675