ELISA/assay

Sheep Interleukin 6 ELISA Kit

MBS738671

48-Strip-Wells

470 USD

ELISA Kit

Sheep Interleukin 6 ELISA Kit

Interleukin 6

interleukin-6; Interleukin-6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); interleukin 6

IL-6

Il6; Il6; ILg6; Ifnb2; Il-6; IL-6

IL6_RAT

Sheep

This assay has high sensitivity and excellent specificity for detection of IL-6. No significant cross-reactivity or interference between IL-6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-6 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL

Immunology

Intended Uses: This IL-6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IL-6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IL-6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-6 antibody and an IL-6-HRP conjugate. The assay sample and buffer are incubated together with IL-6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-6 concentration since IL-6 from samples and IL-6-HRP conjugate compete for the anti-IL-6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-6 from the sample, fewer sites are left to bind IL-6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6 concentration in each sample is interpolated from this standard curve.

7549769

NP_036721.1

NM_012589.2

24,357 Da

Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198529); Cellular Senescence Pathway (1010386); Cellular Responses To Stress Pathway (1010374); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795)

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675