ELISA/assay

Human A Disintegrin And Metalloprotease ELISA Kit

MBS738650

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human A Disintegrin And Metalloprotease ELISA Kit

A Disintegrin And Metalloprotease

A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; COMPase; ADAMTS-7; ADAM-TS 7; metalloprotease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7; COMPase

ADAM

Adamts7; Adamts7; ADAM-TS7; ADAMTS7B; ADAM-TS 7; ADAM-TS7; ADAMTS-7

ATS7_MOUSE

Human

This assay has high sensitivity and excellent specificity for detection of ADAM. No significant cross-reactivity or interference between ADAM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This ADAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: ADAM ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADAM. Standards or samples are then added to the microtiter plate wells and ADAM if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADAM present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADAM are added to each well to "sandwich" the ADAM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADAM and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM concentration in each sample is interpolated from this standard curve.

133778949

NP_001003911.2

NM_001003911.2

177,712 Da

Metabolism Of Proteins Pathway (970583); O-glycosylation Of TSR Domain-containing Proteins Pathway (1000404); O-linked Glycosylation Pathway (1000403); Post-translational Protein Modification Pathway (1000373)

ADAMTS7: Metalloprotease that may play a role in the degradation of COMP. Protein type: EC 3.4.24.-; Secreted; Protease; Cell surface; Motility/polarity/chemotaxis; Secreted, signal peptide. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; cell surface; extracellular region. Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding. Biological Process: proteolysis involved in cellular protein catabolic process; negative regulation of chondrocyte differentiation; proteolysis

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675