ELISA/assay

Porcine Macrophage Inflammatory Protein 1a ELISA Kit

MBS738623

48-Strip-Wells

470 USD

ELISA Kit

Porcine Macrophage Inflammatory Protein 1a ELISA Kit

Macrophage Inflammatory Protein 1a

macrophage inflammatory protein 1 alpha, partial; C-C motif chemokine 3; C-C motif chemokine 3; SIS-beta; PAT 464.1; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a); chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinCleaved into the following chain:MIP-1-alpha(4-69)Alternative name(s):LD78-alpha(4-69)

MIP1a

CCL3; CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha; G0S19-1; MIP1A; SCYA3; MIP-1-alpha

CCL3_HUMAN

Porcine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This MIP-1a ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine MIP-1a. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: MIP-1a ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MIP-1a antibody and an MIP-1a-HRP conjugate. The assay sample and buffer are incubated together with MIP-1a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIP-1a concentration since MIP-1a from samples and MIP-1a-HRP conjugate compete for the anti-MIP-1a antibody binding site. Since the number of sites is limited, as more sites are occupied by MIP-1a from the sample, fewer sites are left to bind MIP-1a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP-1a concentration in each sample is interpolated from this standard

2905628

AAC03539.1

10,085 Da

Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (161020); IL12-mediated Signaling Events Pathway (137922)

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol. Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; protein kinase activity; phospholipase activator activity; CCR5 chemokine receptor binding; chemoattractant activity. Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; behavior; response to toxin; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; regulation of cell shape; negative regulation of bone mineralization; monocyte chemotaxis; cell-cell signaling; positive chemotaxis; calcium ion transport; positive regulation of neuron apoptosis; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; calcium-mediated signaling; MAPKKK cascade; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; osteoblast differentiation; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; cell activation; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

48-Strip-Wells

470 USD

96-Strip-Wells

675