catalog number :
MBS738566
products type :
ELISA Kit
products full name :
Chicken Alanine Aminotransferase ELISA Kit
products short name :
[Alanine Aminotransferase]
other names :
[ALT; ALT; activator of late transcription, experimental; ALT; ALT]
products gene name :
[ALT]
other gene names :
[TP901-1p29]
uniprot entry name :
Q9AZ68_9CAUD
specificity :
This assay has high sensitivity and excellent specificity for detection of ALPI. No significant cross-reactivity or interference between ALPI and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALPI and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Cancer
products description :
Intended Uses: This ALPI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ALPI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ALPI ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALPI antibody and an ALPI-HRP conjugate. The assay sample and buffer are incubated together with ALPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALPI concentration since ALPI from samples and ALPI-HRP conjugate compete for the anti-ALPI antibody binding site. Since the number of sites is limited, as more sites are occupied by ALPI from the sample, fewer sites are left to bind ALPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALPI concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_112692.1
ncbi gb acc num :
NC_002747.1
ncbi mol weight :
16,731 Da
size4 :
10x96-Strip-Wells