Canine Heme Oxygenase 1, Decycling ELISA Kit | MyBioSource MBS738548 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Heme Oxygenase 1, Decycling ELISA Kit

catalog :

MBS738548

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS738548

products type :

ELISA Kit

products full name :

Canine Heme Oxygenase 1, Decycling ELISA Kit

products short name :

Heme Oxygenase 1, Decycling

other names :

heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; heme oxygenase (decyclizing) 1; heme oxygenase (decycling) 1; HSP32

products gene name :

HO1

other gene names :

Hmox1; Hmox1; Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32; HO-1

uniprot entry name :

HMOX1_RAT

reactivity :

Canine

specificity :

This assay has high sensitivity and excellent specificity for detection of HO-1. No significant cross-reactivity or interference between HO-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HO-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This HO-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine HO-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: HO-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HO-1 antibody and an HO-1-HRP conjugate. The assay sample and buffer are incubated together with HO-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HO-1 concentration since HO-1 from samples and HO-1-HRP conjugate compete for the anti-HO-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HO-1 from the sample, fewer sites are left to bind HO-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HO-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

6981032

ncbi acc num :

NP_036712.1

ncbi gb acc num :

NM_012580.2

ncbi mol weight :

33,006 Da

ncbi pathways :

HIF-1 Signaling Pathway (695233); Heme Degradation Pathway (1010848); Iron Uptake And Transport Pathway (1010008); Keap1-Nrf2 Pathway (219759); Metabolism Pathway (1010675); Metabolism Of Porphyrins Pathway (1010846); Mineral Absorption Pathway (212234); Mineral Absorption Pathway (212220); NFE2L2 Pathway (739005); Oxidative Stress Pathway (198512)

ncbi summary :

catalyzes the oxidative cleavage of heme to biliverdin [RGD, Feb 2006]

uniprot summary :

HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family. Protein type: Oxidoreductase; EC 1.14.99.3; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; perinuclear region of cytoplasm; plasma membrane; nucleolus; caveola; cytosol; nucleus. Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; enzyme binding; metal ion binding; phospholipase D activity; heme binding; oxidoreductase activity; heme oxygenase (decyclizing) activity. Biological Process: response to nicotine; cell death; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; heme metabolic process; erythrocyte homeostasis; negative regulation of cell proliferation; regulation of transcription factor activity; heme catabolic process; small GTPase mediated signal transduction; phospholipid metabolic process; regulation of blood pressure; negative regulation of mast cell cytokine production; angiogenesis; negative regulation of neuron apoptosis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; healing during inflammatory response; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; negative regulation of DNA binding; iron ion homeostasis; positive regulation of angiogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; response to hypoxia; response to oxidative stress

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage