Canine Growth Differentiation Factor 11 ELISA Kit | MyBioSource MBS738532 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Growth Differentiation Factor 11 ELISA Kit

catalog :

MBS738532

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS738532

products type :

ELISA Kit

products full name :

Canine Growth Differentiation Factor 11 ELISA Kit

products short name :

Growth Differentiation Factor 11

other names :

growth/differentiation factor 11; Growth/differentiation factor 11; growth/differentiation factor 11; GDF-11; bone morphogenetic protein 11; growth differentiation factor 11; Bone morphogenetic protein 11; BMP-11

products gene name :

GDF11

other gene names :

GDF11; GDF11; BMP11; BMP-11; BMP11; GDF-11; BMP-11

uniprot entry name :

GDF11_HUMAN

reactivity :

Canine

specificity :

This assay has high sensitivity and excellent specificity for detection of GDF-11. No significant cross-reactivity or interference between GDF-11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF-11 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This GDF-11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GDF-11. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cancer

products description :

Principle of the assay: GDF-11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF-11 antibody and an GDF-11-HRP conjugate. The assay sample and buffer are incubated together with GDF-11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF-11 concentration since GDF-11 from samples and GDF-11-HRP conjugate compete for the anti-GDF-11 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF-11 from the sample, fewer sites are left to bind GDF-11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-11 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5031613

ncbi acc num :

NP_005802.1

ncbi gb acc num :

NM_005811.3

ncbi mol weight :

45,091 Da

ncbi summary :

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]

uniprot summary :

GDF11: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q13.2. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: regulation of apoptosis; nervous system development; negative regulation of cell proliferation; negative regulation of cell differentiation; camera-type eye morphogenesis; spinal cord anterior/posterior patterning; ureteric bud development; regulation of MAPKKK cascade; pancreas development; cell maturation; mesoderm development; palate development; cell development; metanephros development; skeletal development; growth

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage