ELISA/assay

Goat Cross-linked C-terminal Telopeptides of type I collagen ELISA Kit

MBS738362

48-Strip-Wells

470 USD

ELISA Kit

Goat Cross-linked C-terminal Telopeptides of type I collagen ELISA Kit

Cross-linked C-terminal Telopeptides of type I collagen

ICTP

Goat

This assay has high sensitivity and excellent specificity for detection of CTx-1. No significant cross-reactivity or interference between CTx-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTx-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CTx-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat CTx-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: CTx-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTx-1 antibody and an CTx-1-HRP conjugate. The assay sample and buffer are incubated together with CTx-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTx-1 concentration since CTx-1 from samples and CTx-1-HRP conjugate compete for the anti-CTx-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTx-1 from the sample, fewer sites are left to bind CTx-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTx-1 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675