ELISA/assay

Porcine Acid Sphingomyelinase ELISA Kit

MBS738210

48-Strip-Wells

470 USD

ELISA Kit

Porcine Acid Sphingomyelinase ELISA Kit

Acid Sphingomyelinase

acid sphingomyelinase; Sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase; acid sphingomyelinase; sphingomyelin phosphodiesterase 1, acid lysosomal; Acid sphingomyelinase; aSMase

ASM

SMPD1; SMPD1; ASM; NPD; ASMASE; ASM; aSMase

ASM_HUMAN

Porcine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Cell Biology

Intended Uses: The AS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: AS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AS antibody and an AS-HRP conjugate. The assay sample and buffer are incubated together with AS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AS concentration since AS from samples and AS-HRP conjugate compete for the anti-AS antibody binding site. Since the number of sites is limited, as more sites are occupied by AS from the sample, fewer sites are left to bind AS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AS concentration in each sample is interpolated from this standard curve.

179095

AAA58377.1

69,624 Da

Ceramide Signaling Pathway (138023); FAS (CD95) Signaling Pathway (138081); Glycosphingolipid Metabolism Pathway (530751); IL2 Signaling Events Mediated By PI3K Pathway (137938); Lysosome Pathway (99052); Lysosome Pathway (96865); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Ovarian Infertility Genes Pathway (198801)

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Phosphodiesterase; EC 3.1.4.12; Lipid Metabolism - sphingolipid. Chromosomal Location of Human Ortholog: 11p15.4-p15.1. Cellular Component: extracellular space; lysosomal lumen; lysosome; plasma membrane; endosome; lamellar body. Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds. Biological Process: response to drug; nervous system development; negative regulation of MAP kinase activity; sphingomyelin metabolic process; sphingolipid metabolic process; positive regulation of apoptosis; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process; response to cocaine; signal transduction; positive regulation of protein amino acid dephosphorylation. Disease: Niemann-pick Disease, Type B; Niemann-pick Disease, Type A

48-Strip-Wells

470 USD

96-Strip-Wells

675