Bovine Glutathione peroxidase ELISA Kit | MyBioSource MBS738198 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Glutathione peroxidase ELISA Kit

catalog :

MBS738198

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS738198

products type :

ELISA Kit

products full name :

Bovine Glutathione peroxidase ELISA Kit

products short name :

[Glutathione peroxidase]

other names :

[glutathione peroxidase; Glutathione peroxidase 3; glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase; glutathione peroxidase 3 (plasma); Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P]

products gene name :

[GSH-Px]

other gene names :

[GPX3; GPX3; GPx-P; GSHPx-3; GSHPx-P; GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P]

uniprot entry name :

GPX3_HUMAN

reactivity :

Bovine

specificity :

This assay has high sensitivity and excellent specificity for detection of GPR77. No significant cross-reactivity or interference between GPR77 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPR77 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

products description :

Intended Uses: This GPR77 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GPR77. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GPR77 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GPR77 antibody and an GPR77-HRP conjugate. The assay sample and buffer are incubated together with GPR77-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPR77 concentration since GPR77 from samples and GPR77-HRP conjugate compete for the anti-GPR77 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPR77 from the sample, fewer sites are left to bind GPR77-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPR77 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

312004115

ncbi acc num :

CAA41228.2

uniprot acc num :

P22352

ncbi mol weight :

25,552 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (941400); Folate Metabolism Pathway (198833); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Oxidative Stress Pathway (198916); Selenium Metabolism And Selenoproteins Pathway (198846)

ncbi summary :

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

uniprot summary :

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family. Protein type: Lipid Metabolism - arachidonic acid; Secreted; EC 1.11.1.9; Secreted, signal peptide; Other Amino Acids Metabolism - glutathione; Oxidoreductase. Chromosomal Location of Human Ortholog: 5q33.1. Cellular Component: extracellular space; extracellular region. Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding. Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; response to lipid hydroperoxide; protein homotetramerization

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage