ELISA/assay

Canine Interleukin 24 ELISA Kit

MBS738189

48-Strip-Wells

470 USD

ELISA Kit

Canine Interleukin 24 ELISA Kit

Interleukin 24

Interleukin 24; Interleukin-24; interleukin-24; melanocyte-associated Mda-7; IL-4-induced secreted protein; melanoma differentiation-associated gene 7 protein; suppression of tumorigenicity 16 (melanoma differentiation); interleukin 24; Melanoma differentiation-associated gene 7 protein; MDA-7; Suppression of tumorigenicity 16 protein

Il-24

IL24; IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B; MDA7; ST16; IL-24; MDA-7

IL24_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of IL-24. No significant cross-reactivity or interference between IL-24 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-24 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This IL-24 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IL-24. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-24 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-24 antibody and an IL-24-HRP conjugate. The assay sample and buffer are incubated together with IL-24-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-24 concentration since IL-24 from samples and IL-24-HRP conjugate compete for the anti-IL-24 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-24 from the sample, fewer sites are left to bind IL-24-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-24 concentration in each sample is interpolated from this standard curve.

16307185

AAH09681.1

7,347 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL23-mediated Signaling Events Pathway (138000); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Senescence And Autophagy Pathway (198780)

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

IL24: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. Belongs to the IL-10 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space; endoplasmic reticulum. Molecular Function: cytokine activity. Biological Process: negative regulation of cell proliferation; wound healing; apoptosis; positive regulation of cell proliferation; positive regulation of caspase activity; positive regulation of JAK-STAT cascade; immune response; negative regulation of caspase activity; inflammatory response; negative regulation of cell migration; serine phosphorylation of STAT3 protein; positive regulation of tyrosine phosphorylation of Stat3 protein

48-Strip-Wells

470 USD

96-Strip-Wells

675