ELISA/assay

Sheep Granulocyte Colony Stimulating Factor ELISA Kit

MBS738137

48-Strip-Wells

470 USD

ELISA Kit

Sheep Granulocyte Colony Stimulating Factor ELISA Kit

Granulocyte Colony Stimulating Factor

granulocyte colony-stimulating factor, partial; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; colony stimulating factor 3 (granulocyte); Pluripoietin; INN: Filgrastim; INN: Lenograstim

GCSF

CSF3; CSF3; GCSF; CSF3OS; C17orf33; C17orf33; GCSF; G-CSF

CSF3_HUMAN

Sheep

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This G-CSF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep G-CSF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: G-CSF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-G-CSF antibody and an G-CSF-HRP conjugate. The assay sample and buffer are incubated together with G-CSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the G-CSF concentration since G-CSF from samples and G-CSF-HRP conjugate compete for the anti-G-CSF antibody binding site. Since the number of sites is limited, as more sites are occupied by G-CSF from the sample, fewer sites are left to bind G-CSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The G-CSF concentration in each sample is interpolated from this standard curve.

297660573

ADI49832.1

18,434 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Malaria Pathway (152665); Malaria Pathway (152657); PI3K-Akt Signaling Pathway (692234)

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Cell cycle regulation; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q11.2-q12. Cellular Component: extracellular space. Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding. Biological Process: positive regulation of myeloid cell differentiation; positive regulation of protein binding; granulocyte differentiation; cytokine and chemokine mediated signaling pathway; multicellular organismal development; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response

48-Strip-Wells

470 USD

96-Strip-Wells

675