ELISA/assay

Porcine Phospholipase A2, Secreted ELISA Kit

MBS738128

48-Strip-Wells

470 USD

ELISA Kit

Porcine Phospholipase A2, Secreted ELISA Kit

Phospholipase A2, Secreted

Phospholipase A2; Phospholipase A2; phospholipase A2; group IB phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; phospholipase A2, group IB (pancreas)<; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B

sPLA2

PLA2G1B; PLA2G1B

PA21B_BOVIN

Porcine

This assay has high sensitivity and excellent specificity for detection of sPLA2. No significant cross-reactivity or interference between sPLA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sPLA2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This sPLA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine sPLA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: sPLA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sPLA2 antibody and an sPLA2-HRP conjugate. The assay sample and buffer are incubated together with sPLA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sPLA2 concentration since sPLA2 from samples and sPLA2-HRP conjugate compete for the anti-sPLA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by sPLA2 from the sample, fewer sites are left to bind sPLA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sPLA2 concentration in each sample is interpolated from this standard curve.

11467959

P00593.2

16,002 Da

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48-Strip-Wells

470 USD

96-Strip-Wells

675