catalog number :
MBS738026
products type :
ELISA Kit
products full name :
Porcine Tenascin C ELISA Kit
products short name :
Tenascin C
other names :
tenascin-C, partial; Tenascin-C; Tenascin-C
uniprot entry name :
Q99857_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of TNC. No significant cross-reactivity or interference between TNC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TNC and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
products categories :
Neurobiology
products description :
Intended Uses: This TNC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine TNC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay TNC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TNC antibody and an TNC-HRP conjugate. The assay sample and buffer are incubated together with TNC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNC concentration since TNC from samples and TNC-HRP conjugate compete for the anti-TNC antibody binding site. Since the number of sites is limited, as more sites are occupied by TNC from the sample, fewer sites are left to bind TNC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNC concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB47574.1
ncbi mol weight :
9,764 Da