ELISA/assay

Monkey Substance P ELISA Kit

MBS738011

48-Strip-Wells

470 USD

ELISA Kit

Monkey Substance P ELISA Kit

Substance P

substance P; Protachykinin-1; protachykinin-1; PPT; tachykinin 1; Tachykinin (substance P, neurokinin A, neuropeptide K, neuropeptide gamma); tachykinin, precursor 1; PPTCleaved into the following 5 chains:Substance P; Neurokinin A; NKAAlternative name(s):Neuromedin L; Substance K

S.P

Tac1; Tac1; TAC; PPTA3; Ppt5fl; RATPPTA3; Nka; Nkna; Tac2; NKA; NPK

TKN1_RAT

Monkey

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

Intended Uses: This SP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SP. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This SP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the Assay: SP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SP antibody and an SP-HRP conjugate. The assay sample and buffer are incubated together with SP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SP concentration since SP from samples and SP-HRP conjugate compete for the anti-SP antibody binding site. Since the number of sites is limited, as more sites are occupied by SP from the sample, fewer sites are left to bind SP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SP concentration in each sample is interpolated from this standard curve.

56068

CAA39752.1

P06767

11,374 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); G Alpha (q) Signalling Events Pathway (1010197); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1010213); Peptide Ligand-binding Receptors Pathway (1010165); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162); Tachykinin Receptors Bind Tachykinins Pathway (1010167)

encodes neuropeptides substance P, neurokinin A, neuropeptide K, and neuropeptide gamma [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675