ELISA/assay

Mouse A Proliferation inducing ligand ELISA Kit

MBS738004

48-Strip-Wells

470 USD

ELISA Kit

Mouse A Proliferation inducing ligand ELISA Kit

A Proliferation inducing ligand

APRIL; Tumor necrosis factor ligand superfamily member 13; tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2; tumor necrosis factor (ligand) superfamily, member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD_antigen: CD256

APRIL

TNFSF13; TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715; APRIL; TALL2; ZTNF2; UNQ383/PRO715; APRIL; TALL-2; TRDL-1

TNF13_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of APIL. No significant cross-reactivity or interference between APIL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between APIL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1ng/mL

Immunology

Intended Uses: This APIL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse APIL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: APIL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APIL antibody and an APIL-HRP conjugate. The assay sample and buffer are incubated together with APIL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APIL concentration since APIL from samples and APIL-HRP conjugate compete for the anti-APIL antibody binding site. Since the number of sites is limited, as more sites are occupied by APIL from the sample, fewer sites are left to bind APIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APIL concentration in each sample is interpolated from this standard curve.

24934972

AAL90442.1

36,589 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Gene Expression Pathway (105937); HuR Stabilizes MRNA Pathway (187216); Intestinal Immune Network For IgA Production Pathway (128760); Intestinal Immune Network For IgA Production Pathway (128670); Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway (187211); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269); Spinal Cord Injury Pathway (739007)

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol; external side of plasma membrane. Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding. Biological Process: immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; gene expression; signal transduction; positive regulation of isotype switching to IgA isotypes

48-Strip-Wells

470 USD

96-Strip-Wells

675