Monkey Cx3CL1/Fractalkine ELISA Kit | MyBioSource MBS738002 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Cx3CL1/Fractalkine ELISA Kit

catalog :

MBS738002

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS738002

products type :

ELISA Kit

products full name :

Monkey Cx3CL1/Fractalkine ELISA Kit

products short name :

Cx3CL1/Fractalkine

other names :

CX3CL1; CX3CL1 protein; fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); chemokine (C-X3-C motif) ligand 1; CX3CL1 protein

products gene name :

Cx3CL1

other gene names :

CX3CL1; CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin

uniprot entry name :

Q6I9S9_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of CX3CL1. No significant cross-reactivity or interference between CX3CL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CX3CL1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This CX3CL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CX3CL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

. Principle of the assay: CX3CL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CX3CL1 antibody and an CX3CL1-HRP conjugate. The assay sample and buffer are incubated together with CX3CL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX3CL1 concentration since CX3CL1 from samples and CX3CL1-HRP conjugate compete for the anti-CX3CL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX3CL1 from the sample, fewer sites are left to bind CX3CL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX3CL1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

48146969

ncbi acc num :

CAG33707.1

uniprot acc num :

Q6I9S9

ncbi mol weight :

42,187 Da

ncbi pathways :

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Direct P53 Effectors Pathway (137939); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114)

uniprot summary :

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: extracellular space; cell surface; integral to membrane; plasma membrane; extracellular region. Molecular Function: protein binding; chemokine activity; receptor binding. Biological Process: positive regulation of transforming growth factor-beta1 production; neutrophil chemotaxis; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; chemotaxis; angiogenesis involved in wound healing; macrophage chemotaxis; leukocyte adhesive activation; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage