Sheep Pulmonary surfatcant-associated protein B ELISA Kit | MyBioSource MBS737898 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sheep Pulmonary surfatcant-associated protein B ELISA Kit

catalog :

MBS737898

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS737898

products type :

ELISA Kit

products full name :

Sheep Pulmonary surfatcant-associated protein B ELISA Kit

products short name :

Pulmonary surfatcant-associated protein B

other names :

pulmonary surfactant-associated protein B; Pulmonary surfactant-associated protein B; pulmonary surfactant-associated protein B; 6 kDa protein; 18 kDa pulmonary-surfactant protein; pulmonary surfactant-associated proteolipid SPL(Phe); surfactant protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe)

products gene name :

SP-B

other gene names :

SFTPB; SFTPB; SP-B; PSP-B; SFTB3; SFTP3; SMDP1; SFTP3; SP-B

uniprot entry name :

PSPB_HUMAN

reactivity :

Sheep

sequence length :

393

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This SPB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep SPB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: SPB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SPB antibody and an SPB-HRP conjugate. The assay sample and buffer are incubated together with SPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPB concentration since SPB from samples and SPB-HRP conjugate compete for the anti-SPB antibody binding site. Since the number of sites is limited, as more sites are occupied by SPB from the sample, fewer sites are left to bind SPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPB concentration in each sample is interpolated from this standard curve. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

ncbi gi num :

288856299

ncbi acc num :

NP_000533.3

ncbi gb acc num :

NM_000542.3

uniprot acc num :

P07988

ncbi mol weight :

42,117 Da

ncbi summary :

This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]

uniprot summary :

SFTPB: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. Defects in SFTPB are the cause of pulmonary surfactant metabolism dysfunction type 1 (SMDP1); also called pulmonary alveolar proteinosis due to surfactant protein B deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid- Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPB are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS). RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called hyaline membranes . A variation Ile to Thr at position 131 influences the association between specific alleles of SFTPA1 and respiratory distress syndrome in premature infants. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2p12-p11.2. Cellular Component: extracellular space; lysosome. Biological Process: organ morphogenesis; sphingolipid metabolic process; respiratory gaseous exchange. Disease: Surfactant Metabolism Dysfunction, Pulmonary, 1

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage