ELISA/assay

Bovine Gastric Inhibitory Polypeptide ELISA Kit

MBS737888

48-Strip-Wells

470 USD

ELISA Kit

Bovine Gastric Inhibitory Polypeptide ELISA Kit

Gastric Inhibitory Polypeptide

gastric inhibitory polypeptide preproprotein; Gastric inhibitory polypeptide; gastric inhibitory polypeptide; incretin hormone; glucose-dependent insulinotropic polypeptide; gastric inhibitory polypeptide; Glucose-dependent insulinotropic polypeptide; Incretin hormone

GIP

GIP; GIP; GIP

GIP_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of GIP. No significant cross-reactivity or interference between GIP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GIP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This GIP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GIP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: GIP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GIP antibody and an GIP-HRP conjugate. The assay sample and buffer are incubated together with GIP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GIP concentration since GIP from samples and GIP-HRP conjugate compete for the anti-GIP antibody binding site. Since the number of sites is limited, as more sites are occupied by GIP from the sample, fewer sites are left to bind GIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GIP concentration in each sample is interpolated from this standard curve.

4758436

NP_004114.1

NM_004123.2

17,108 Da

Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Insulin Secretion Pathway (777534); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Signal Transduction Pathway (477114)

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q21.3-q22. Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region. Molecular Function: hormone activity. Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; response to lipid; sensory perception of pain; signal transduction; response to amino acid stimulus; response to organic cyclic substance; endocrine pancreas development; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion

48-Strip-Wells

470 USD

96-Strip-Wells

675