ELISA/assay

Porcine Trypsinogen activation peptide ELISA Kit

MBS737816

48-Strip-Wells

470 USD

ELISA Kit

Porcine Trypsinogen activation peptide ELISA Kit

Trypsinogen activation peptide

TAP, partial; Antigen peptide transporter 2; antigen peptide transporter 2; ABC transporter, MHC 2; peptide supply factor 2; peptide transporter PSF2; really interesting new gene 11 protein; transporter 2, ABC (ATP binding cassette); peptide transporter involved in antigen processing 2; ATP-binding cassette, sub-family B (MDR/TAP), member 3; transporter 2, ATP-binding cassette, sub-family B (MDR/TAP); ATP-binding cassette sub-family B member 3; Peptide supply factor 2; Peptide transporter PSF2; PSF-2; Peptide transporter TAP2; Peptide transporter involved in antigen processing 2; Really interesting new gene 11 protein

TAP

TAP2; TAP2; APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E; ABCB3; PSF2; RING11; Y1; APT2; PSF-2

TAP2_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of TAP. No significant cross-reactivity or interference between TAP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TAP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This TAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine TAP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: TAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TAP antibody and an TAP-HRP conjugate. The assay sample and buffer are incubated together with TAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TAP concentration since TAP from samples and TAP-HRP conjugate compete for the anti-TAP antibody binding site. Since the number of sites is limited, as more sites are occupied by TAP from the sample, fewer sites are left to bind TAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAP concentration in each sample is interpolated from this standard curve.

1217589

BAA07665.1

72,003 Da

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); Adaptive Immune System Pathway (366160); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); ER-Phagosome Pathway (477124); Herpes Simplex Infection Pathway (377873)

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]

TAP2: a transporter associated with antigen processing (TAP) protein. Member of the ATP binding cassette (ABC) family of transmembrane transporters. Transports peptides across the endoplasmic reticulum membrane for assembly of major histocompatibility complex class I molecules. Two subunits, TAP1 and TAP2, are required for peptide transport. ATP hydrolysis is important for transport activity. Nascent MHC class I molecules associate with TAP via tapasin. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: endoplasmic reticulum membrane; TAP complex; membrane; integral to membrane; integral to endoplasmic reticulum membrane. Molecular Function: tapasin binding; protein binding; peptide transporter activity; TAP1 binding; transporter activity; peptide antigen-transporting ATPase activity; ATP binding. Biological Process: intracellular transport of viral proteins in host cell; antigen processing and presentation of peptide antigen via MHC class I; metabolic process; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; peptide antigen transport; antigen processing and presentation of endogenous peptide antigen via MHC class I; transmembrane transport; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; cytosol to ER transport; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent. Disease: Bare Lymphocyte Syndrome, Type I

48-Strip-Wells

470 USD

96-Strip-Wells

675