Canine Interferon beta ELISA Kit | MyBioSource MBS737661 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Interferon beta ELISA Kit

catalog :

MBS737661

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS737661

products type :

ELISA Kit

products full name :

Canine Interferon beta ELISA Kit

products short name :

[Interferon beta]

products name syn :

[Canine Interferon b ELISA Kit]

other names :

[interferon beta; Interferon beta; interferon beta; IFN-beta; fibroblast interferon; interferon, beta 1, fibroblast; Fibroblast interferon]

products gene name :

[IFN-beta]

products gene name syn :

[IFN-b]

other gene names :

[IFNB1; IFNB1; IFB; IFF; IFNB; IFB; IFNB; IFN-beta]

uniprot entry name :

IFNB_HUMAN

reactivity :

Canine

specificity :

This assay has high sensitivity and excellent specificity for detection of IFNW1. No significant cross-reactivity or interference between IFNW1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFNW1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Reactivity Note: No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.

products categories :

Cancer

products description :

Intended Uses: This IFNW1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken IFNW1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IFNW1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFNW1 antibody and an IFNW1-HRP conjugate. The assay sample and buffer are incubated together with IFNW1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNW1 concentration since IFNW1 from samples and IFNW1-HRP conjugate compete for the anti-IFNW1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNW1 from the sample, fewer sites are left to bind IFNW1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNW1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4504603

ncbi acc num :

NP_002167.1

ncbi gb acc num :

NM_002176.2

ncbi mol weight :

22,294 Da

ncbi pathways :

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833)

uniprot summary :

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to virus; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; cell surface receptor linked signal transduction; negative regulation of viral genome replication; B cell differentiation; positive regulation of innate immune response; defense response to bacterium; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; B cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage