ELISA/assay

Bovine Retinol Binding Protein 4 ELISA Kit

MBS737578

48-Strip-Wells

470 USD

ELISA Kit

Bovine Retinol Binding Protein 4 ELISA Kit

Retinol Binding Protein 4

retinol-binding protein 4; Retinol-binding protein 4; retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; retinol binding protein 4, plasma; Plasma retinol-binding protein; PRBP; RBP

RBP4

Rbp4; Rbp4; RBPA; PRBP; RBP

RET4_RAT

Bovine

This assay has high sensitivity and excellent specificity for detection of RBP4. No significant cross-reactivity or interference between RBP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RBP4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ?g/mL.

Intended Uses: This RBP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine RBP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: RBP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RBP4 antibody and an RBP4-HRP conjugate. The assay sample and buffer are incubated together with RBP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RBP4 concentration since RBP4 from samples and RBP4-HRP conjugate compete for the anti-RBP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by RBP4 from the sample, fewer sites are left to bind RBP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RBP4 concentration in each sample is interpolated from this standard curve.

158187535

NP_037294.1

NM_013162.1

23,220 Da

Disease Pathway (1010484); Diseases Associated With Visual Transduction Pathway (1010532); Retinoid Cycle Disease Events Pathway (1010533); Retinoid Metabolism And Transport Pathway (1010244); Retinol Metabolism Pathway (219784); Signal Transduction Pathway (1010015); The Canonical Retinoid Cycle In Rods (twilight Vision) Pathway (1010245); Visual Phototransduction Pathway (1010243)

may play a role in vitamin A transport [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675