catalog number :
MBS737528
products type :
ELISA Kit
products full name :
Goat Cross-linked N-terminal Telopeptides of type I collagen ELISA Kit
products short name :
Cross-linked N-terminal Telopeptides of type I collagen
other names :
NTX, partial; NTX; NTX
products gene name :
INTP
uniprot entry name :
Q9QTG7_CBDP
specificity :
This assay has high sensitivity and excellent specificity for detection of NTx-1. No significant cross-reactivity or interference between NTx-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NTx-1 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This NTx-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat NTx-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cell Biology
products description :
Principle of the assay: NTx-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for NTx-1. Standards or samples are then added to the microtiter plate wells and NTx-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of NTx-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for NTx-1 are added to each well to "sandwich" the NTx-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NTx-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTx-1 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
BAA75084.1
ncbi mol weight :
146,743 Da