catalog number :
MBS737435
products type :
ELISA Kit
products full name :
Canine Adipocyte Fatty Acid Binding Protein ELISA Kit
products short name :
Adipocyte Fatty Acid Binding Protein
other names :
A-FABP, partial; A-FABP; A-FABP
products gene name :
A-FABP
uniprot entry name :
A9YWQ7_ANAPL
specificity :
This assay has high sensitivity and excellent specificity for detection of AFABP. No significant cross-reactivity or interference between AFABP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AFABP and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This AFABP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine AFABP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cardiovascular
products description :
Principle of the assay: AFABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AFABP antibody and an AFABP-HRP conjugate. The assay sample and buffer are incubated together with AFABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AFABP concentration since AFABP from samples and AFABP-HRP conjugate compete for the anti-AFABP antibody binding site. Since the number of sites is limited, as more sites are occupied by AFABP from the sample, fewer sites are left to bind AFABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFABP concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ABY27083.1
ncbi mol weight :
3,816 Da