ELISA/assay

Canine Sex Hormone Binding Globulin ELISA Kit

MBS737318

48-Strip-Wells

470 USD

ELISA Kit

Canine Sex Hormone Binding Globulin ELISA Kit

Sex Hormone Binding Globulin

sex hormone-binding globulin isoform 1; Sex hormone-binding globulin; sex hormone-binding globulin; sex steroid-binding protein; testosterone-binding beta-globulin; testosterone-estrogen-binding globulin; testosterone-estradiol-binding globulin; testis-specific androgen-binding protein; sex hormone-binding globulin; Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP; Testosterone-estradiol-binding globulin; TeBG; Testosterone-estrogen-binding globulin

SHBG

SHBG; SHBG; ABP; SBP; TEBG; SHBG; SBP; ABP; TeBG

SHBG_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of SHBG. No significant cross-reactivity or interference between SHBG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SHBG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 nmol/L.

Intended Uses: This SHBG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine SHBG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Priciple of the assay: SHBG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SHBG antibody and an SHBG-HRP conjugate. The assay sample and buffer are incubated together with SHBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SHBG concentration since SHBG from samples and SHBG-HRP conjugate compete for the anti-SHBG antibody binding site. Since the number of sites is limited, as more sites are occupied by SHBG from the sample, fewer sites are left to bind SHBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SHBG concentration in each sample is interpolated from this standard curve.

7382460

NP_001031.2

NM_001040.4

41,712 Da

Nongenotropic Androgen Signaling Pathway (138048)

This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

SHBG: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: extracellular region. Molecular Function: androgen binding. Biological Process: primary spermatocyte growth

48-Strip-Wells

470 USD

96-Strip-Wells

675